Saturday, January 31, 2009

Starting a small research lab

Having done microscopy work for 5 years, and investing much time and money studying my own fluids, I have decide to extend my research practices to include samples from other subjects.

I have already done this on a small level. I feel there is much that can be accomplished by running routine, as well as complex tests on subjects who complain of chronic symptoms. Subjects must have been previously evaluated by standard medical tests, which have failed to elucidate any obvious problems.
Some of the tests I would like to do are as follows; HLA determination, cytokine tests, blood morphology in native samples, antigen screening of intestinal fluids and urine, peptide screening and documentation, protein isolation from aforementioned fluids and development of data base of random protein samples, and toxicity tests for body fluids to name a few.
I would like to venture into hybridoma technology [some day] and do work with monoclonal antibodies.
There are many things to consider when designing a lab.
First, you must have an appropriate location, there must be equipment and supplies, modifications like vent-hoods, disposal protocols, safety protocols, lab wear, incubation station [vents] etc.
Since I am doing this on a small budget, the location will be quite small.
I plan to have a person in the lab doing work everyday, so students will need to be considered, and small compensation awarded. This will be stressful on budget, so donations to the lab is a must.

Therefore small advertising campaigns and circulation in health-concerned events to distribute business cards, and generate interest must be part of my job.
The idea, is to build a facility which is interactive with ordinary people. This is a way to help individuals with their own research and give them vital information they need to help themselves.
Another feature of the lab, will be consultation.
I would like to share the space with a health expert such as nutritional consultant. This way, there can be co-operation between different modes of information.
The key feature of the lab will be high performance microscopy, and micro graphic documentation. I plan on using this feature to collaborate with professionals in the field, who will receive a fee for reviewing micrograph's, other data.
I would also like to have a network of other labs on file known for specialties in various disciplines.

Thursday, January 29, 2009

Understanding the behaviour of my parasite

One of the interesting characteristics of my coccidian parasite are the mother cells. They "bud" which I find unusual. These are seen here to the right. The twin oocysts [not seen here] are opaque and contain spores only [gamatocytes?]

All attempts to transfer the original oocysts to fresh medium have failed. I have used many different mediums, and have settled on purified beef liver extract with 30% bleach, and 20% lysol disinfectant. This medium could not sustain the oocysts for long. However, the bradyzoites [for lack of term] multiplied to fill the supernatant significantly. Bacteria have been controlled by addition of above mentioned chemicals.
A mystery exists, because the original sample still sustains "twin" oocysts, and bradyzoites have settled into nests without over contaminating rest of solution.

Sunday, January 25, 2009

Understanding the nature of coccidian parasites

From studies done on plasmodium and toxoplasma gondii to name a few, I find intracellular parasites are a marvel of nature. They can literally cause subtle genetic manipulations in host physiology, and in some cases psychology. My own microscopic observations, demonstrate this parasite has a complexity beyond many microbes in nature, and it's survivability will ensure it's place in human epidimiology for some time to come.
I am looking for other scientists or even hobbiests interested in these microbes, for continued discussion and exchange of scientific data.
I myself am infected with a unique type, and will dedicate the rest of my life to understanding this parasite. I have microscopic data available for viewing, and will post them on occasion.
That is it for now,
umm