Thursday, January 29, 2009

Understanding the behaviour of my parasite

One of the interesting characteristics of my coccidian parasite are the mother cells. They "bud" which I find unusual. These are seen here to the right. The twin oocysts [not seen here] are opaque and contain spores only [gamatocytes?]

All attempts to transfer the original oocysts to fresh medium have failed. I have used many different mediums, and have settled on purified beef liver extract with 30% bleach, and 20% lysol disinfectant. This medium could not sustain the oocysts for long. However, the bradyzoites [for lack of term] multiplied to fill the supernatant significantly. Bacteria have been controlled by addition of above mentioned chemicals.
A mystery exists, because the original sample still sustains "twin" oocysts, and bradyzoites have settled into nests without over contaminating rest of solution.

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