Apicomplexia complexity

I suspect there are cross-gene species of apicomplexans that may be worth looking at, in certain clinical situations.
I think there needs to be expansion of available primers for spp. such as plasmodium.

Staining for fecal oocysts averaging at 3-4 micrometers should be emphasized in stool tests of patients with autoimmune symptoms.

New apicomplexan\other parasite?

I have some interesting information to reveal "soon" regarding some intensive DNA sequencing of my parasite which was submitted weeks ago. Information from my own documentation of this microbe is in earlier postings.

This information will interest those studying apicomplexia, their genetic diversity and ability for quroum sensing.

My Microscopic research studio is now open for business in Toronto.

Continuation of culture evaluation

A typical cluster.






This one is weird it has interesting internal morphology.
I have seen others without pigmentation, and many moving entities inside the membrane. If I see more like those, I will post video.






Typical cell. I can't even tell if it grows from the "rosegerms" shown in previous post's micrographs.




These micrographs show developing structures, that I admit, were unexpected, and completely unexplainable, at least so far.
I have made some adjustments to the growth medium, by infusing carbon dioxide into the broth.
These structures may be producing a pigment, because I do not remember contaminating with stains. The structures are reddish-orange, and look like they could be attempting to assemble a larger organism.
This does exist in nature, but usually from decimated multicellular life-forms.
Anyway, I did not see these in initial scans, now they are everywhere.
I was expecting cell clusters to form, but the medium is different from the one that supported these cell groups. However, there are cellular organisms developing in this medium as well, and I have posted a micrograph of one of them below.

Continuing with this strange microbe

Small cluster of rosegerms embeded in host proteins


Possible development of complex phase cell structures


Interesting structural development.


Notice the consistency among structures.


Same structures


Look at them closely. These are also absorbing cytoplasmic stain, that accidentally got into broth solution.



All micrographs can be enlarged by clicking on image.




I am following this latest microbe, as described in former postings, because it has a interesting and predictable development in vitro. It is from a chronically afflicted individual, taken from puss sample.
I determined anecdotally, that because the lesion was spontaneous, and without external injury, the same possible agent causing systemic problem may be found in puss.
It turns out, that I did indeed find an interesting microbe amongst the self induced defense proteins. These proteins continue to be processed long after the immune cells have degenerated. It would seem these are the host's natural antibiotics. They even resemble protein clouds formed from penicillin. I can tell they are human by distinct odour [a slight cheese smell]. Obviously these molecules are aromatic in structure.

The curious thing about this sample which is being documented, there are phases which eventually result in the differentiation of cells. I am going to take everyone through this.
The sample was obtained from dried out specimen in petri dish.
It was placed in distilled water with vitamins, amino acids, minerals and butric acid.
Then placed in small tube in incubator.
I did initial scans, which I placed on youtube [see profile page for links].
It showed the extent of biofilm production from activated "host" proteins. The density had increased to high levels, then condensed, and settled.
Now I am posting micrographs of the microbe as it develops, in real time situation.
This way, my predictions can be scrutinized, and seen from other perspectives.

I am calling the microbe "rosegerm", until I can ID it. As seen in micrographs, it starts as a small rose like structure, and progresses to become larger, and has spores being produced in them [it appears so]. As they enlarge, the formation of cell structure appears. In later date, we may see entire cell clusters.
I think this may be a fungal phenotype of microsporidium [but please don't hold me to that]!
I will post every now and again, as the culture matures.

Micrographs of suspect microbes

Here are some interesting micrographs--plus one video of microbes developed from sample of person with chronic problems.
This particular sample was taken from puss, however, the same microbes emerged from blood smear [both samples were treated in process which I developed for assembling problematic microbes originating in body fluids]. I rely on the assumption the microbes utilize advanced system of quorum sensing.
I have my share of critics, I can assure you.

The first micrograph is a mature cell. It makes things, then becomes empty, with a few granules left.

The second photo shows colony of cell types. I don't know what they are, but were assembled from free moving proteins\microbes which start as type of biofilm.
The biofilms are observable throughout solution, but only some become active.
The cells that develop, are varied. Some show protozoan type activity, others move slightly, and others interact. I had attempted to grow these in medium designed for fungi, but nothing fungal grew. I plan to repeat this excercise, with control.

The third micrograph shows more colony cells but shows differences among cell morphology.

The last video, shows the essence of the "bee swarm" clouds I have described as observing. [these are stationary] I have decided this is chemical activity, likely as a defense mechanism. It is reminiscent of mammalian granulocytes.

Swarming germs

Unfortunately, the clip shown is too small for good viewing.
If you look to the far left terminal of the cell cluster, you will see movement. This activity is from microbes being released from cells, where they will join up with others to form hives. From these hives, individual microbes with join up with others in free solution, where they will form swarms.