Sunday, May 17, 2009

Continuing with this strange microbe


Small cluster of rosegerms embeded in host proteins


Possible development of complex phase cell structures


Interesting structural development.


Notice the consistency among structures.


Same structures


Look at them closely. These are also absorbing cytoplasmic stain, that accidentally got into broth solution.



All micrographs can be enlarged by clicking on image.




I am following this latest microbe, as described in former postings, because it has a interesting and predictable development in vitro. It is from a chronically afflicted individual, taken from puss sample.
I determined anecdotally, that because the lesion was spontaneous, and without external injury, the same possible agent causing systemic problem may be found in puss.
It turns out, that I did indeed find an interesting microbe amongst the self induced defense proteins. These proteins continue to be processed long after the immune cells have degenerated. It would seem these are the host's natural antibiotics. They even resemble protein clouds formed from penicillin. I can tell they are human by distinct odour [a slight cheese smell]. Obviously these molecules are aromatic in structure.

The curious thing about this sample which is being documented, there are phases which eventually result in the differentiation of cells. I am going to take everyone through this.
The sample was obtained from dried out specimen in petri dish.
It was placed in distilled water with vitamins, amino acids, minerals and butric acid.
Then placed in small tube in incubator.
I did initial scans, which I placed on youtube [see profile page for links].
It showed the extent of biofilm production from activated "host" proteins. The density had increased to high levels, then condensed, and settled.
Now I am posting micrographs of the microbe as it develops, in real time situation.
This way, my predictions can be scrutinized, and seen from other perspectives.

I am calling the microbe "rosegerm", until I can ID it. As seen in micrographs, it starts as a small rose like structure, and progresses to become larger, and has spores being produced in them [it appears so]. As they enlarge, the formation of cell structure appears. In later date, we may see entire cell clusters.
I think this may be a fungal phenotype of microsporidium [but please don't hold me to that]!
I will post every now and again, as the culture matures.

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To view youtube account my user name is mualfa123 This will enable you to see clips of my microscopic work.