Legally Kidnapped: Unlawful confinement and abduction of students by school officials

Legally Kidnapped: Unlawful confinement and abduction of students by school officials

parasites

I am blogging again. I am going to investigate the types of intracellular parasites infecting many poeple with chronic illnesses or irritants.
I will examine stool specimens if peope bring them to me. I can record findings with microscopic equipment.

Treating toxo and malaria with herbicides?

Could herbicide fluridone be used as therapeutic treatment of certain apicomplexans infecting humans?

My new office

These are pics of my new microscopy mini lab. I am open for biz!

My Apologies

I am in the process of setting up an office in the research district of Toronto. I will be open for business very soon, and will except appointments.
Will be networking with other researchers, so opportunities will exist to find answers, at least, to some of the pressing questions about acquired chronic physical symptoms in healthy people.
My focus will be on immunology, and pathological consequences of self verses non-self antigens.

Apicomplexia complexity

I suspect there are cross-gene species of apicomplexans that may be worth looking at, in certain clinical situations.
I think there needs to be expansion of available primers for spp. such as plasmodium.

Staining for fecal oocysts averaging at 3-4 micrometers should be emphasized in stool tests of patients with autoimmune symptoms.

New apicomplexan\other parasite?

I have some interesting information to reveal "soon" regarding some intensive DNA sequencing of my parasite which was submitted weeks ago. Information from my own documentation of this microbe is in earlier postings.

This information will interest those studying apicomplexia, their genetic diversity and ability for quroum sensing.

My Microscopic research studio is now open for business in Toronto.

Continuation of culture evaluation

A typical cluster.






This one is weird it has interesting internal morphology.
I have seen others without pigmentation, and many moving entities inside the membrane. If I see more like those, I will post video.






Typical cell. I can't even tell if it grows from the "rosegerms" shown in previous post's micrographs.




These micrographs show developing structures, that I admit, were unexpected, and completely unexplainable, at least so far.
I have made some adjustments to the growth medium, by infusing carbon dioxide into the broth.
These structures may be producing a pigment, because I do not remember contaminating with stains. The structures are reddish-orange, and look like they could be attempting to assemble a larger organism.
This does exist in nature, but usually from decimated multicellular life-forms.
Anyway, I did not see these in initial scans, now they are everywhere.
I was expecting cell clusters to form, but the medium is different from the one that supported these cell groups. However, there are cellular organisms developing in this medium as well, and I have posted a micrograph of one of them below.

Continuing with this strange microbe

Small cluster of rosegerms embeded in host proteins


Possible development of complex phase cell structures


Interesting structural development.


Notice the consistency among structures.


Same structures


Look at them closely. These are also absorbing cytoplasmic stain, that accidentally got into broth solution.



All micrographs can be enlarged by clicking on image.




I am following this latest microbe, as described in former postings, because it has a interesting and predictable development in vitro. It is from a chronically afflicted individual, taken from puss sample.
I determined anecdotally, that because the lesion was spontaneous, and without external injury, the same possible agent causing systemic problem may be found in puss.
It turns out, that I did indeed find an interesting microbe amongst the self induced defense proteins. These proteins continue to be processed long after the immune cells have degenerated. It would seem these are the host's natural antibiotics. They even resemble protein clouds formed from penicillin. I can tell they are human by distinct odour [a slight cheese smell]. Obviously these molecules are aromatic in structure.

The curious thing about this sample which is being documented, there are phases which eventually result in the differentiation of cells. I am going to take everyone through this.
The sample was obtained from dried out specimen in petri dish.
It was placed in distilled water with vitamins, amino acids, minerals and butric acid.
Then placed in small tube in incubator.
I did initial scans, which I placed on youtube [see profile page for links].
It showed the extent of biofilm production from activated "host" proteins. The density had increased to high levels, then condensed, and settled.
Now I am posting micrographs of the microbe as it develops, in real time situation.
This way, my predictions can be scrutinized, and seen from other perspectives.

I am calling the microbe "rosegerm", until I can ID it. As seen in micrographs, it starts as a small rose like structure, and progresses to become larger, and has spores being produced in them [it appears so]. As they enlarge, the formation of cell structure appears. In later date, we may see entire cell clusters.
I think this may be a fungal phenotype of microsporidium [but please don't hold me to that]!
I will post every now and again, as the culture matures.

Micrographs of suspect microbes

Here are some interesting micrographs--plus one video of microbes developed from sample of person with chronic problems.
This particular sample was taken from puss, however, the same microbes emerged from blood smear [both samples were treated in process which I developed for assembling problematic microbes originating in body fluids]. I rely on the assumption the microbes utilize advanced system of quorum sensing.
I have my share of critics, I can assure you.

The first micrograph is a mature cell. It makes things, then becomes empty, with a few granules left.

The second photo shows colony of cell types. I don't know what they are, but were assembled from free moving proteins\microbes which start as type of biofilm.
The biofilms are observable throughout solution, but only some become active.
The cells that develop, are varied. Some show protozoan type activity, others move slightly, and others interact. I had attempted to grow these in medium designed for fungi, but nothing fungal grew. I plan to repeat this excercise, with control.

The third micrograph shows more colony cells but shows differences among cell morphology.

The last video, shows the essence of the "bee swarm" clouds I have described as observing. [these are stationary] I have decided this is chemical activity, likely as a defense mechanism. It is reminiscent of mammalian granulocytes.

Swarming germs

Unfortunately, the clip shown is too small for good viewing.
If you look to the far left terminal of the cell cluster, you will see movement. This activity is from microbes being released from cells, where they will join up with others to form hives. From these hives, individual microbes with join up with others in free solution, where they will form swarms.

PCR results are in

I have sent the specimen being documented in this blog, for mtDNA analysis, and the preliminary results are in. It is unknown at this time. They ran queries for fungal sequences and it came up negative. Many, who are familiar with microbiology have suggested it may be fungal in origin. This would be natural assumption, judging by morphology, as seen in micrograph above.
It is apicomplexan, but classification may be a task ahead. The DNA is still available for further sequencing, which may have to be done.
Is it novel? Well, it certainly has not been well documented. Since people like myself are infected with this or variations of this, as a human disease I would have expected a faster hit.

Documenting curious microbe. Pathogen?

Click on image for closer scrutinity


I have isolated a very interesting microbe, and have photographed it through high performance camera at special settings under dark field at 600+-x.
The microbe is motile, and tends to roll. It is made of smaller subunits, which I never would have seen without the computer images. The subunits are green, with red on polar end. I have observed the units break off and migrate away from mother unit. The unit, which I call "sponge integer" has been cultivated from sample placed in 30% bleach with purified sterile water and incubated at 37+ degrees C. The sample was blood obtained from microscopic slide wet mount.
The donor, has multiple acquired symptomologies without definitive diagnosis. Suspicion of bartonella has been noted by specialist, but I do not see any indications of this.

If you study the image, you can see the surrounding subunits, these are found throughout sample. There are also other ameoboid type microbes in the solution, and scattered biofilms.
I speculate the red terminals are acidic molecular residues, and the green is neutral PH. This type of configuration is noted in microbes I have observed with intracellular parasites. I am not jumping to conclusions here, but feel these microbes are worthy of recording for future reference.

More on parasites

I have been trying to upload some important clips, but they would take.

Two cells at high magnification

The first photo is from my "oocysts". The fact these are occysts will be confirmed soon through mtDNA analysis. It is not fungal [unless recombinant], because sporozoits were grown and observed, and even infected other cells---check old posts for documentation on that. Sorry, the photo was taken by commercial camera, but it is high magnification. It is at same setting as second micrograph shown.


This second micrograph is a very high magnification of 2 cells from on going culture of unknown spores. There has been much activity between metabolites, microbes etc., in solution, and the appearance of cells which I say could be oocysts. But yeast cells, and penicillium molds have also been isolated from original source in dry plate culture. I have compared the spores from the {obtained} fungal specimens to objects in free solution broth. From observation, I have eliminated those as being the source of growing bodies in solution, which I believe are parasites.

The cells in micrograph look close to cells I have isolated from my own body, but are from individual hundreds of miles away, whom I have never even met.

Immature microbes, becoming something weird

Click on image for close up
The first micrograph is taken under normal light setting, shows flat view of microbe. [BTW this microbe glides]

The second is under dark field, the light refracts, so there is limited clarity. The colors are very close, though. There is methylene blue in the solution, which causes some of this effect. The energy of the microbe, does emit color. It was seen moving, before capture. They do not live long under glass.

The yeast in question

This was the yeast grown directly from slide sample, although I isolated 3 strains of penicillum as well. The yeast was pure white.
The cells making up the penicillium had some similarities with the growing cells [in pic 3 posts down from here] but the ones in question, seem to be different.
I would have guessed yeast right off the bat, but the microbe in question seems to be directly associated with these cells. I am almost sure they are sporozoites, and if this thought is not swayed, I will suggest to donor, that we have it analyzed genetically.

Check out the suspect oocysts in previous photo's and take note of the cell wall. It has definite thickness--it is a different cell.

More micrographs

These 3 micrographs are as follows: 1) bio film seen as typical film in sample [stained with methylene blue] it appears almost 3D under direct observation, with the stained section in top layer 2) typical bio film, with heavy globulins 3)oocysts[?] which are under investigation--stained with MB. It is not surprising the suspect oocysts stained heavy. This was seen in oocysts from my personal specimen sample obtained from diarrhea contained entirely in mucus.
These oocysts[?] developed from blood specimen of person with chronic symptomology.

Preliminary micrograph of suspect microbe

Click on image for size increase. [4-5 um dark field]

This is just a quick peek at what I "suspect" is a participant in the process which results in the emergence of oocysts. This one is later stage, and there are small proteins tethered to one end.
In the host, the first observation from blood, looked like a rod bacteria. In later experiments, this same ron type bacteria became present. Other forms resembling this form, had proteins which moved in and conjugated with this structure. It becomes covered over time, and starts to produce globulin structures. This is a far as I get.
There are also cysts in same solution, so to much going on for me to make any kind of conclusions.

Click on image for larger picture.

This micrograph is from a sample which contained strange, non-bacterial microbe, which shows signs of complex organization. I have gone through great lengths to promote differentiation of microbe into other stages of development. This compares to trying to make a caterpiller make a chrysalis, and become a butterfly!
Being lead by intuition, some scientific method, and experience, I eventually came up with these structures, which I think are the oocysts made by unknown microbes.
Any comments from experienced microbiologists will be most welcome.