Results from experiment

I wish these microbes were easier to predict. The anticipated infection of cells was not accomplished as expected. There were a few parameters in the experiment which may have altered the outcome.
First, the chemicals in the specimen supernatant [bleach, lysol disinfectant] had affects on the saliva derived cells. The cells massively amplified production of antimicrobial proteins.
The initial observation, before incubation showed 'zoites infiltrate cells. They did this slowly, by attaching to surface of membrane, and eventually slipping to the interior portion of membrane. Then, proceeded to central part of cell.
Predictable you say?
The incubation mix, showed significant drop in number of 'zoites. The amount of protein products from cells, increased a thousand fold. Many cells were lysed, and the cells that were infected, had active granulation which was making life difficult for the 'zoites.
I conclude, that these cells are not suitable housing for the 'zoites but that cellular infection is definitly the objective of these microbes.
It also demonstrates why I have problems with dehydration, and depression of eletrolytes. The cells use up salts, and other resources for the constant generation of cells, and their chemical products.
The million dollar question is; what cells are being infiltrated, and which defense mechanism is showing ineffective action against these 'zoites?
I have included a short "clip" showing infected cell, in conflict with intruding 'zoites. This is at 600-700x mag under dark field conditions. The visual information is not the best [waiting for expensive Lumenera camera to arrive], and there are no annotations. You might have to squint a bit. The 'zoites are orangeish red, and the background movement is chemical action from cell. the activity is observed at lower
left of cell.

My apologies, the transfer of clip was not as clear as expected.

Experiment to infiltrate cells with 'zoites

If you place saliva in a test tube, cells will continue to reproduce. These cells will protect themselves against invasion from microbes through TLR's and the production of basic antimicrobial proteins. They need very little attention to conditions governing growth.
Since the parasitic 'zoites [term used until formally classified] seek out cells for housing, and cannot survive antibodies, I think these cells should provide the perfect model for examining the interaction of the cell invasion process. I will place the infective agent into small tube with cells, and incubate for 12 hours. I will report in next post, with photo's.