Saturday, February 7, 2009

Securing indentification of ambiguous microbes

Today I want to talk about the options I am considering for proper identification of my parasite.
This is important, because there is no doubt that this organism is important in the investigation of digestive and other problematic human health issues.
It is also advantageous for developing testing modalities.

The best test for identifying this microbe is called "COI Barcode" [cytochrome oxidase] which is a protein coding region of mitochondrial DNA {mtDNA}.
The area of the gene, the first half containing approximately 648 base pairs, is the easiest to process thus the cheapest.
Here are 3 main reasons to use COI barcode.
1) easiest to recover, including degraded sources from diverse taxa
2)readily aligned for sequence comparisons
3)effective for making distinctions between closely related species including both vertebrate and invertebrate taxa.

Six practical reasons for using this barcode:
1) Works with small traces. Barcoding can identify species from "bits and pieces" including material in foodstuffs

2) Works for all stages of life. Barcoding can identify species in all it's many forms, from eggs and seeds, through larva and seedlings to adults and flowers.

3)Unmasks look-alikes. Barcoding can distinguish among species that look the same, uncovering dangerous organisms that look the same as harmless and enabling a more accurate view of biodiversity.

4) Reduces ambiguity. A barcode provides an unambiguous digital identifying feature for quantifying species, supplementing more analog gradations of words, shapes and colors.

5) makes expertise go further. Scientists can equip themselves with barcoding to speed identification of unknown organisms and facilatate rapid recognition of new species.

6) Democratizes access. A standard library of barcodes will empower many more people to callby name, the species around them.

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