Monday, March 30, 2009
Quorum sensing and "hive" behavior in microbes.
I am writing based on my own experience with this. I know there is a whole science that is based on pleomorphism, but I have been seeing something, I think is unique.
I have covered quite a bit of ground with my own parasite, and have "almost" established, that it belongs to the family of apicomplexia. This is based on stages I have extrapolated by isolation, growth and observation [DNA analysis pending].
I recently observed blood sample of 'subject' who has multiple symptoms including neurological and digestive issues.
Upon first observation of blood on slide [week-old, but sealed] there was evidence of "spore" like objects in the [1-2 micrometer range dark field] sample.
The neutrophils were releasing granules, [granule proteins abundant] and I think there MAY have been NK cells present. I did not see macrophages, other than the neutro-leukocytes. The leukocytes were loaded up with these spores. It is obvious from this, that an immunotolerance has been established to a certain point. The housekeeping cells, however, are in full force trying to control extracellular infiltration of these spores. There were a few bacteria present, but I could not be sure of contamination.
The 'subject' is chronically affected, but still maintains relative health.
This person had western blot for Lyme disease, and was positive for a few bands, which I reviewed, and decided was not enough for solid positive. It did show many elements of bacterial antigens.
I would have like to seen results for protozoan antigens--------and this is where I am going.
I cultivated the blood in saline solution, at body temp. This activated the spores, which turned out to be small short-rod bacteria. They created aggregates or "clusters" which had a very interesting pattern, shown by spectral rings in dark field. Many were independent, and there was an increase of very small proteins. This could have been host proteins, but many more spores were developing.
As these clusters increased, I started to notice [after a few days] yeast-like cells growing in chains of 4. There were singlets, triplets, quins, and more, but the vast majority were quads.
These cells would eventually migrate together, and create a larger unit, something like the smaller bacterial looking things were doing.
This reminded me of the behavior I had observed with my oocysts! I therefore believe these are indeed oocysts. But the morphology is remarkably phenotypic of yeast cells.
The sample does not grow on plate nutrient with sugar, and microbes are temperature sensitive, going into dormancy outside 37 C.
I am speculating, there is a fungal quality to these microbes, as well as protozoan. The small microbes, look and behave like my own 'zoites.
I will be analyzing the spectral rings, and comparing them to my 'zoites with special equipment soon.
I think we have another example of these mystery apicomplexan-like parasites.
More tests are now being conducted, and I will post more on this later.
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Myxobacteria
ReplyDeleteThank you very much anonymous, this could provide a vital lead for me.
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